Instrument

The three centers are equipped and offer access for a cryo-electron microscope Titan G4 (Thermo Fisher Scientific)

Technical specification :

The Titan Krios G4 is the latest generation of electron microscopes for the observation of biological specimens at high resolution. It benefits from the latest technologies for improved contrast, as well as the latest generation of direct electron detectors (Falcon4i). Its high stability is very well adapted to long, high resolution data recording sessions, in a fully automated manner.

  • Microscope: ThermoFisher Scientific Titan Krios G4
  • Accelerating voltage = 300 kV
  • Electron source: Cold Field Emission Gun
  • Fringe free illumination
  • Detector: Falcon 4i camera
  • Energy filter: Selectris X
  • Phase plate: FEI Volta Phase Plate
  • Cs = 2.7 mm
  • Pixel size
MagnificationCalibrated pixel size (Å)
270 kx0,46
215 kx0,57
165 kx0,73
130 kx0,94
105 kx1,2
  • Movies format: eer or tiff
  • Acquisition software: EPU or TOMO 5 (for Grenoble and SOLEIL sites) SERIAL EM for Strasbourg site
  • Both remote and on-site sessions are possible.